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KMID : 1188320100040040488
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Gut and Liver
2010 Volume.4 No. 4 p.488 ~ p.497
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Near-Infrared Fluorescence Imaging Using a Protease-Specific Probe for the Detection of Colon Tumors
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Yoon Soon-Man
Myung Seung-Jae
Ye Byong-Duk
Kim In-Wha
Lee Nam-Gon
Ryu Yeon-Mi
Park Kyeong-Soon
Kim Kwang-Meyung
Kwon Ick-Chan
Park Young-Soo
Park Chan-Sik
Moon Dae-Hyuk
Kim Do-Hoon
Do Mi-Young
Byeon Jeong-Sik
Yang Suk-Kyun
Kim Jin-Ho
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Abstract
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Background/Aims: Early tumor detection is crucial for the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging using a target-activatable probe may permit earlier disease detection. Matrix metalloproteinases (MMPs) participate in tumorigenesis and tumor growth. The aim of this study was to determine whether NIRF imaging using an MMP-activatable probe can detect colon tumors at early stages.
Methods: We utilized two murine colon cancer models: a sporadic colon cancer model induced by azoxymethane (AOM), and a colitis-associated cancer model induced by a combination of AOM and dextran sodium sulfate (DSS). Colonic lesions were analyzed by histologic examination, Western blotting, immunohistochemical staining, and NIRF imaging using an MMP-activatable probe.
Results: Multiple variable-sized tumors developed in both models and progressed from adenomas to adenocarcinomas over time. At the early stage of the AOM/DSS model, diffuse inflammation was observed within the tumors. MMP expression increased progressively through normal, inflammation, adenoma, and adenocarcionoma stages. NIRF signal intensities were strongly correlated with each tumor stage from adenoma to adenocarcinoma. NIRF imaging also distinguished tumors from inflamed mucosa.
Conclusions: NIRF imaging using a protease-activatable probe may be a useful tool for early tumor detection. This approach could translate to improve the endoscopic detection of colon tumors, especially in patients with inflammatory bowel disease.
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KEYWORD
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Colon cancer, Inflammatory bowel disease, Near-infrared fluorescence, Matrix metalloproteinases
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